595 In vitro evaluation of skin distribution and human dermal papilla cells proliferation for minoxidil 5% topical lotion hydrogel

For decades, the treatment of reference for androgenetic alopecia has been topical application minoxidil 2% or 5%, which is commercially available as a solution and foam. When compounded, may be incorporated in variable strengths to base order meet individual needs men. An alternative compounded formulation was developed include proprietary hydrogel that alcohol-free reduce skin irritation, does not require pH adjustment facilitate compounding process. In vitro studies were conducted investigate proliferation human dermal papilla cells upon with 5% lotion hydrogel. Additional evaluated distribution across skin. The commercial product used positive control. analysis, bromodeoxyuridine staining assay identified proliferating 24 hours exposure by (commercial versus formulation), using fluorescence detection. effect significant both (RFU 181,446 ±38,171, p=0.021) 183,109±24,416, p=0.006), when compared negative control (untreated cells). distribution, Franz Skin Finite Dose Model percutaneous absorption into dermis receptor medium samples from two male Caucasian donors. Following integrity test 3 diffusion, extracted layer analyzed UPLC. It shown similar products. performance comparable reference. As such, easy compound, promising therapeutic alopecia.